Optimized determination of lycopene in canine plasma using reversed-phase high-performance liquid chromatography.

摘要: Abstract An isocratic high-performance liquid chromatographic method with detection at 472 nm was developed, optimized and validated for the determination of lycopene in canine plasma. Ethyl-β-apo-8′-carotenoate used as internal standard. A Hypersil BDS RP-C18 column (150 mm × 4.6 mm), 5 μm particle size, equilibrated a mobile phase composed acetonitrile methanol (50:50, v/v). Its flow rate 1.5 ml/min. The elution time ethyl-β-apo-8′-carotenoate approximately 11 5 min, respectively. Calibration curves were linear concentration range 3–200 ng/ml Limits quantification plasma 1 4 ng/ml, Recovery greater than 97%. Intra- inter-day relative standard deviation less 1.8 3.1%, This applied to levels after single dose administration dogs.