All-D-cecropin B: synthesis, conformation, lipopolysaccharide binding, and antibacterial activity.

摘要: Cecropin B (LCB) is a natural peptide with antibacterial and antifungal properties. The enantiomer of LCB, containing all-D amino acids (DCB), was synthesized to examine its binding conformation DCB compared by circular dichroism. Both the L- D-peptides showed an identical induction α-helical secondary structure. However, studies between Lipopolysaccharide (LPS) or LCB were studied dimethylmethylene blue spectrophotometric assay, showing two enantiomeric peptides differed in their interaction LPS. Antibacterial activity determined against three Gram-negative bacteria, Pantoea agglomerans (ATCC 27996), Escherichia coli 8739), Pseudomonas aeruginosa 17648), giving comparable results LCB.