Laser-induced microbubble poration of localized single cells.
作者： Qihui Fan , Wenqi Hu , Aaron T. Ohta
DOI: 10.1039/C3LC51394G
关键词:
摘要: Laser-induced microbubbles were used to porate the cell membranes of localized single NIH/3T3 fibroblasts. Microsecond laser pulses focused on an optically absorbent substrate, creating a vapour microbubble that oscillated in size at focal point fluidic chamber. The shear stress accompanying bubble oscillation was able nearby cells. Cell poration demonstrated with delivery FITC-dextran dye various molecular weights. Under optimal conditions, efficiency up 95.2 ± 4.8%, while maintaining 97.6 2.4% viability. system is target without disturbing surrounding
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