Gelatin nonwovens-based epithelial morphogenesis involves a signaling axis comprising EGF-receptor, MAP kinases ERK 1/2, and β1 integrin.

摘要: In biomaterials research, biomechanics which support tissue regeneration steadily gains of importance. Hence, we have previously shown that gelatin-based electrospun nonwoven mats (NWMs) with a distinct modulus elasticity (3.2 kPa) promotes epithelial morphogenesis. Since molecular mechanisms this morphogenesis are still unknown, the present study aims at identifying molecules, involved herein. Epithelia established on NMWs showed persistence activated state epidermal growth factor receptor (EGF-R), phosphorylated src-specific tyrosine 845 (EGF-RT845 ) throughout observation period 10 days. To elucidate whether observed mechanistically involves EGF-R signaling, inhibited EGF-R, by employing EGF-RT845 specific inhibitor Gefitinib (IRESSA®). administration yielded reduced expression β1 integrin subunit, well-known cell-matrix interaction receptor, concomitant downregulation p42/44 ERK1/2 MAP-kinase activity. is -dependent or emerging from exposed epithelia, grown NWMs, ERK1/2-directed U0126. absence Gefitinib, inhibition activity resulted in decreased protein levels, thus indicating dependent and not . Our results first time an EGF-R-β1 integrin-signaling axis, including ERK1/2, NWM-elasticity-based © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 663-677, 2019.