Kinetics of adsorption of lysozyme at the air–water interface and the role of protein charge

摘要: Abstract The adsorption kinetics of hen egg white lysozyme at the air–water interface has been studied using specular neutron reflectometry. Experiments were performed a number pH values to examine effect charge on rate protein adsorption. Solutions in air Contrast matched water 1 mg/mL made. These allow direct determination surface excess protein. High repetition experiments, with short collection times, used accurately determine only excess—derived from product film thickness and scattering length density layer. kinetic traces where is charged are well fitted by first-order equation two linear regions, change gradient occurs as concentration reaches steady state. This behaviour characteristic transport distortion molecules, followed rearrangement equilibrium function state concentrations reaching 1.4 mg m −2 4 3 mg m 11. Protein inversely related dependency explored through thermodynamic analysis.