Understanding type 1 diabetes through proteomics.

摘要: ABSTRACTIntroduction: Auto-immunity against pancreatic beta-cells leads to an absolute shortage of the hormone insulin, resulting in hyperglycemia and onset type 1 diabetes (T1D). Proteomic approaches have been used elucidate mechanisms beta-cell dysfunction death.Areas covered: In present review, we discuss discoveries proteome that contributed better insights role T1D. Techniques, such as 2D-DIGE MALDI imaging, together with new for sample preparation, including laser capture microdissection immunopeptidomics, resulted novel mechanistic pathogenesis We describe how proteomic studies lines well isolated islets from animal models humans discovered intracellular signaling pathways leading destruction, generation neo-antigens through post-translational modifications antigens biomarkers disease progres...