Characterization of RET proto-oncogene 3' splicing variants and polyadenylation sites: a novel C-terminus for RET.

摘要: The RET proto-oncogene, which encodes a receptor tyrosine kinase, displays multiple alternative splicing variants. Splicing of sequences 3' exon 19 to generate several coding and untranslated region (UTR) has been previously reported. We have sequenced the full length characterized transcripts UTRs generated by terminus. These analyses were performed using both cDNA cloned from pheochromocytoma library reverse transcriptase PCR products RNA neuroblastoma cell line (LA-N-2). Three different carboxyl termini identified. In addition nine 51 terminal amino acid forms already known, we identified third with 43 acids predicted encode novel protein isoform. A total 3621 base pairs DNA 19, spans alternatively spliced exons UTRs, was sequenced. Four polyadenylation sites observed combinations sequence suggest that up 10 40 may exist.