[44] Determination of molecular species of rod outer segment phospholipids
作者： Rex D. Wiegand , Robert E. Anderson
DOI: 10.1016/S0076-6879(82)81046-X
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摘要: Publisher Summary This chapter discusses the determination of molecular species rod outer segment (ROS) phospholipids. The total phospholipid fraction is resolved into individual lipid classes by two-dimensional thin-layer chromatography (TLC). Two studies ROS have indicated that they are rather unique because contain large proportions dipolyunsaturated species. a class calculated to best fit estimate using proportional analysis, algebraic manipulations, and normalization all data from diglyceride acetates (DGAC) their fatty acid composition distribution. Positional analysis acids performed subjecting each phospholipase A 2 hydrolysis, which specifically releases 2-position. for DGAC mixture accomplished with pancreatic lipase hydrolysis. results will be quantitative chemical description major phospholipids found in segments.
sci-hub.se 参考文章(18)
B.J. HOLUB, A. KUKSIS, Metabolism of molecular species of diacylglycerophospholipids. Advances in lipid research. ,vol. 16, pp. 1- 125 ,(1978) , 10.1016/B978-0-12-024916-9.50007-X
MI de Aveldaño Caldironi, NG Bazan, Acyl Groups, Molecular Species, and Labeling by 14C-Glycerol and 3H-Arachidonic Acid of Vertebrate Retina Glycerolipids Function and Biosynthesis of Lipids. ,vol. 83, pp. 397- 404 ,(1977) , 10.1007/978-1-4684-3276-3_37
Robert E. Anderson, Maureen B. Maude, Lipids of ocular tissues. 8. The effects of essential fatty acid deficiency on the phospholipids of the photoreceptor membranes of rat retina. Archives of Biochemistry and Biophysics. ,vol. 151, pp. 270- 276 ,(1972) , 10.1016/0003-9861(72)90497-3
Robert E. Anderson, Linda Sperling, Lipids of ocular tissues: VII. Positional distribution of the fatty acids in the phospholipids of bovine retina rod outer segments Archives of Biochemistry and Biophysics. ,vol. 144, pp. 673- 677 ,(1971) , 10.1016/0003-9861(71)90374-2
William R. Morrison, Lloyd M. Smith, Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride–methanol Journal of Lipid Research. ,vol. 5, pp. 600- 608 ,(1964) , 10.1016/S0022-2275(20)40190-7
George Rouser, A. N. Siakotos, Sidney Fleischer, Quantitative analysis of phospholipids by thin-layer chromatography and phosphorus analysis of spots. Lipids. ,vol. 1, pp. 85- 86 ,(1966) , 10.1007/BF02668129
Robert E. Anderson, Martin Risk, Lipids of ocular tissues IX. The phospholipids of frog photoreceptor membranes Vision Research. ,vol. 14, pp. 129- 131 ,(1974) , 10.1016/0042-6989(74)90127-8
A. Kuksis, L. Marai, Determination of the complete structure of natural lecithins. Lipids. ,vol. 2, pp. 217- 224 ,(1967) , 10.1007/BF02532559
F. E. Luddy, R. A. Barford, S. F. Herb, P. Magidman, R. W. Riemenschneider, Pancreatic lipase hydrolysis of triglycerides by a semimicro technique Journal of the American Oil Chemists' Society. ,vol. 41, pp. 693- 696 ,(1964) , 10.1007/BF02661412
Randall Wood, Fred Snyder, Tumor lipids: Metabolic relationships derived from structural analyses of acyl, alkyl, and alk-1-enyl moieties of neutral glycerides and phosphoglycerides Archives of Biochemistry and Biophysics. ,vol. 131, pp. 478- 494 ,(1969) , 10.1016/0003-9861(69)90420-2