A+U content rather than a particular nucleotide order determines the specificity of RNase E cleavage.

摘要: Abstract Ribonuclease E has a central role in Escherichia coli mRNA decay and is dependent on functional product of the rne (also called ams or hmp1) gene. We investigated requirements for RNase cleavage by introducing random mutations into decanucleotide region at 5' end pACYC184 RNA I studying effects these position rne-dependent vivo E-mediated cutting vitro. find that precise point can be altered specifically reproducibly sequence changes cleaved and, therefore, not determined distance measured nucleotides from any other secondary structure I. Although occurs within sequences rich A and/or U affected extent continuity regions cleaved, there no simple relationship between order phosphodiester bond cleaved. Thus, our results are consistent with either notion cleavages consensus contrary view few primary structural constraints than preference cleaving to an AU dinucleotide.