Direct Bisulfite Sequencing and Methylation Specific PCR to Detect Methylation of p15INK4b and F7 genes in Coronary Artery Disease Patients
作者： R. Mirfakhraie , A. Movafagh , A. Sayad , K. Amini , M. Yari
DOI: 10.22059/JSCIENCES.2016.56005
关键词:
摘要: Genome-Wide Association Studies (GWAS) have identified genetic variants contributing to the risk of cardiovascular disease (CVD) at chromosome 9p21 locus. The is an important susceptibility locus for several multifactorial diseases like ischemic stroke, aortic aneurysm, type 2 diabetes mellitus and coronary artery (CAD). F7 gene because its role in activating extrinsic pathway by exposure tissue factor after plaque disruption related atherothrombosis. aim present study was evaluate methylation status two CAD genes, p15INK4b F7, Iranian patients with Thirty samples from 15 male female diagnosed 3 vessels 60 Samples non-CAD controls who underwent angiography analyzed MSP direct sequencing. DNA levels increased significantly comparison control group (p-value<0.001). To quantitative analysis methylation, bisulfite sequencing method revealed 6 methylated CpGs islands. No significant difference promoter observed between group. Based on findings this it possible assume, associated pathophysiology CAD.
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