[17] Chemical synthesis of fructose 2,6-bisphosphate
作者： S.J. Pilkis , M.R. El-Maghrabi , D.A. Cumming , J. Pilkis , T.H. Claus
DOI: 10.1016/S0076-6879(82)89019-8
关键词:
摘要: Publisher Summary This chapter describes the chemical synthesis of fructose 2,6-bisphosphate. Fructose 2,6-bisphosphate is an allosteric activator 6-phosphofructo-1-kinase and inhibitor 1,6-bisphosphatase. The method based on 2-phosphate. treatment 1-phosphate with dicyclohexylcarbodiimide (DCC) in aqueous pyridine leads to formation cyclic 1,2-phosphate. Alkaline hydrolysis that compound yields 2-phosphate as one product other. Base-catalyzed ring opening 1,2-cyclic 6-bisphosphate (II) both 1,6-bisphosphate destroyed by heating alkali, whereas stable purified anion-exchange chromatography. 6-Phosphofructo-1- kinase activity activation 2,6- bisphosphate are determined aldolase-coupled assay. Purified rat liver used overall yield this limited primarily base-catalyzed reaction, but key purification alkali.
sci-hub.se 参考文章(11)
K. Uyeda, E. Furuya, A.D. Sherry, The structure of “activation factor” for phosphofructokinase. Journal of Biological Chemistry. ,vol. 256, pp. 8679- 8684 ,(1981) , 10.1016/S0021-9258(19)68897-6
S.J. Pilkis, M.R. El-Maghrabi, J. Pilkis, T. Claus, Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-bisphosphate. Journal of Biological Chemistry. ,vol. 256, pp. 3619- 3622 ,(1981) , 10.1016/S0021-9258(19)69494-9
S.J. Pilkis, M.R. El-Maghrabi, J. Pilkis, T.H. Claus, D.A. Cumming, Fructose 2,6-bisphosphate. A new activator of phosphofructokinase. Journal of Biological Chemistry. ,vol. 256, pp. 3171- 3174 ,(1981) , 10.1016/S0021-9258(19)69584-0
E Van Schaftingen, L Hue, H G Hers, Fructose 2,6-bisphosphate, the probably structure of the glucose- and glucagon-sensitive stimulator of phosphofructokinase. Biochemical Journal. ,vol. 192, pp. 897- 901 ,(1980) , 10.1042/BJ1920897
T.H. Claus, J. Schlumpf, J. Pilkis, R.A. Johnson, S.J. Pilkis, Evidence for a new activator of rat liver phosphofructokinase. Biochemical and Biophysical Research Communications. ,vol. 98, pp. 359- 366 ,(1981) , 10.1016/0006-291X(81)90848-2
Fred R. Seymour, Roger D. Knapp, Judith E. Zweig, Stephen H. Bishop, 13C-nuclear magnetic resonance spectra of compounds containing β-D-fructofuranosyl groups or residues Carbohydrate Research. ,vol. 72, pp. 57- 69 ,(1979) , 10.1016/S0008-6215(00)83923-3
Carolyn Sue Richards, Kosaku Uyeda, Changes in the concentration of activation factor for phosphofructokinase in hepatocytes in response to glucose and glucagon Biochemical and Biophysical Research Communications. ,vol. 97, pp. 1535- 1540 ,(1980) , 10.1016/S0006-291X(80)80040-4
S. Pilkis, J. Schlumpf, J. Pilkis, T.H. Claus, Regulation of phosphofructokinase activity by glucagon in isolated rat hepatocytes Biochemical and Biophysical Research Communications. ,vol. 88, pp. 960- 967 ,(1979) , 10.1016/0006-291X(79)91501-8
Emile VAN SCHAFTINGEN, Henri-Géry HERS, Formation of Fructose 2,6-Bisphosphate from Fructose 1,6-Bisphosphate by Intramolecular Cyclisation followed by Alkaline Hydrolysis FEBS Journal. ,vol. 117, pp. 319- 323 ,(1981) , 10.1111/J.1432-1033.1981.TB06339.X
Emile Van Schaftingen, Henri-Géry Hers, Synthesis of a stimulator of phosphofructokinase, most likely fructose 2,6-bisphosphate, from phosphoric acid and fructose 6-phosphoric acid Biochemical and Biophysical Research Communications. ,vol. 96, pp. 1524- 1531 ,(1980) , 10.1016/0006-291X(80)91347-9