Apoptosis and expression of inducible nitric oxide synthase are mutually exclusive in renal mesangial cells.

摘要: Nitric oxide (NO) is a multipurpose messenger molecule, important for blood vessel relaxation, neuronal communication, and antimicrobial activities. The generation of NO from L-arginine catalyzed by synthase (NOS). An inducible form NOS, iNOS, was first characterized in macrophages then many other tissues cells, including renal mesangial cells. Mesangial cells play crucial role the regulation glomerular filtration rate as well pathophysiology certain forms glomerulonephritis which produce high amounts. Because reports have associated production with apoptotic cell death we recently demonstrated NO-mediated apoptosis searched relationship between situ iNOS induction immunocytochemistry terminal desoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining. RAW 264.7 exhibited homogeneous expression nuclei iNOS-containing upon stimulation interferon-gamma lipopolysaccharide. In contrast, stimulated rat stained heterogeneously depending on passage iNOS-stimulating pathway. expressing did not display signs and, vice versa, showing characteristic features stain iNOS. Thus, our study suggests that react to interleukin-1 and/or cAMP-elevating compounds mutually exclusive responses, either or undergoing programmed death.