Microarray-based survey of a subset of putative olfactory genes in the mosquito Anopheles gambiae.

摘要: Female Anopheles gambiae mosquitoes respond to odours emitted from humans in order find a blood meal, while males are nectar feeders. This complex behaviour is controlled at several levels, but probably initiated by the interaction of various molecules antennal sensilla. Important early odour recognition events include odourant binding proteins (OBPs), which may be involved molecule transport, receptors (ORs) that expressed chemosensory neurones and degrading enzymes (ODEs). To obtain better understanding expression patterns genes host reception females, we generated custom microarray study their steady state mRNA levels tissues, antennae palps. These results were supported quantitative RT PCR. Our detected OBPs significantly higher palps females vs. males, others showed opposite pattern. Most slightly down-regulated 24 h after feeding, some, especially those with up-regulated blood-fed suggesting shift human seeking feeding.