Improved detection and quantitation of norovirus from water

摘要: Norovirus is associated commonly with human sewage and responsible for numerous cases of waterborne foodborne gastroenteritis every year. Assays using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) have been developed norovirus, however, accurate detection quantitation remain problematic owing to a lack suitable positive controls. To improve enumeration norovirus genomes from water, synthetic genogroup II standard competitive internal control were developed. The demonstrates identical amplification efficiency as wildtype can be used viral surrogate in labs restricted access norovirus. quantifies sample inhibition, allowing complex environmental samples. Seawater samples spiked or bird guano evaluated the assay part methods comparison study. Inhibition was detected nine 36 (25%) samples, two which proved upon re-analysis. Results support specificity this human-source (sewage) fecal contamination. Overall, use signify great advance over traditional controls suggest that molecular techniques analysis could become standardized routine water quality monitoring.