Modeling Microbial Virulence in a Genomic Era: Impact of Shared Genomic Tools and Data Sets

摘要: This chapter discusses the relevance of model host pathogenesis as a third general approach to studying microbial virulence, in which infections are studied context nonvertebrate whole animal hosts. The explains combination four key steps: (i) development host-pathogen system, (ii) genomic tools both pathogen and host, (iii) distribution use these by greater research community beyond laboratories involved their initial development, (iv) collection ultimate integration experimental data from wide variety groups made possible widespread common resource its accompanying Web-accessible public database. Pseudomonas aeruginosa PA14 was chosen for construction non-redundant mutant library because it is remarkably virulent greatest number hosts tested, well murine systems infection. There major advantages opposed traditional screening random strains avirulent or attenuated mutants. A microarray experiment Caenhorhabidits elegans defective newly defined defense response gene identified candidate downstream genes important pathogens.