Suppression of Growth-Associated Phosphorylation of Proteins of Fibroblasts by Collagen Fibrils
作者： Norimasa Kosekt , Hajime Sato , Katsutoshi Yoshizato
DOI: 10.3109/15419069609081023
关键词:
摘要: Collagen fibrils suppressed serum- or epidermal growth factor (EGF)-inducible DNA synthesis of human fibroblasts. The phosphorylation cellular proteins upon these mitogenic stimulation was analyzed by two-dimensional polyacrylamide gel electrophoresis in order to reveal a possible interference collagen with the signal transduction pathway coupled protein phosphorylation-dephosphorylation reaction. Spots phosphorylated numbered 192 on plain plastic which were reduced 143 fibrils. More than half them matched between two substrates, most much more weakly EGF stimulated cells plastic. Among an approximate molecular weight 27K and isoelectric point 5.3 early highly responsive EGF, seemed be catalyzed mainly kinase C tyrosine kinase. significantly this phosphorylation. present study demonstrates that modulate growth-associated cells, seems lead suppression synthesis.
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