Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen.

摘要: Microscopic identification of pollen morphological phenotypes has been the traditional method used to identify and quantify collected by air monitoring stations worldwide. Although this enabled a semi-standardized approach for assessment exposure, limitations including labor intensiveness, required expertise, examiner bias, inability differentiate species, genera, in some cases families have limited data derived from these stations. Recent advances chemical, biochemical molecular detection methods provided standardized alternatives microscopic approach. In review, we examine applicability alternative methodologies, particular nucleic acid based assays involving quantitative polymerase chain reaction, airborne pollen.