Gastric GISTs: Analysis of c-Kit, PDGFRA and BRAF mutations in relation to prognosis and clinical pathological characteristics of patients – A GIRCG study

摘要: Abstract Background Gastric gastrointestinal stromal tumors (GISTs) represent a subgroup of GISTs with better prognosis than those located in other areas. In this retrospective study we performed molecular characterization large series patients gastric relation to clinical–pathological characteristics and prognosis. Methods DNA was extracted from paraffin-embedded sections 221 GIST submitted surgery. Exons 9, 11, 13 17 KIT , exons 12 18 PDGFRA 11 15 BRAF were analyzed by direct sequencing. Cox regression analysis adjusted for factors evaluate mutations the composite endpoint relapse or death. Results observed 119 (53.8%) 56 (25.3%) patients, respectively, whereas 46 (20.8%) had wild type (wt) disease. Univariable analyses showed that high Miettinen risk category presence ulceration deletions associated increased death (p  remained an independent prognostic factor (HR adj  = 2.65, 95% CI [1.15–6.13], p = 0.023). Moreover, exon codons 557, 558 559 higher wt  = 3.29 [1.64–6.64], p = 0.001). Conclusions especially involving 559, correlated more aggressive phenotype