Predicting outcome based on minimal residual disease (MRD) using multiparameter flow cytometry (FC) in acute myeloid leukemia (AML) patients (Pts).

摘要: 6565 Background: Most AML pts will experience relapse caused by persistent leukemic cells during complete remission (CR). The aim of our study was to predict outcome in CR assessing their MRD using standard 4-colour FC panels available at institute. Methods: We queried database between 1/2004 10/2010 for newly diagnosed untreated >18 years age who achieved after one induction regimen. Treatment included 7+3 or similar intensive approaches. gating strategy used a series Boolean regions that best defined the diagnostic abnormal population based on its expression patterns three (F7-CD38,CD10,CD19,CD34; F9-C11b,CD33,CD13,CD34; F38-CD15,CD56,CD7,CD34). same were applied post-induction samples, and number residual events determined. Results: 140 patients with median 64 (range 24-93) years, including 74 females (52.8%) analyzed; 67 (47.8%) relapsed. Eighty-eight (62.8%) CD34-po...