Expression of fibronectin splicing variants in organ transplantation: a differential pattern between rat cardiac allografts and isografts.

摘要: Allograft rejection is associated with infiltration of inflammatory cells and deposition extracellular matrix proteins. The extent to which diversity in the regulates cell function transplants remains unclear. One group proteins, termed fibronectins (FNs), exhibits inherent as a consequence alternative splicing three segments: EIIIA, EIIIB, or V. Although EIIIA segment has documented functions mesenchymal differentiation, neither this nor EIIIB have been tested for effects specific leukocyte functions. By contrast, V region can include CS-1 leukocytes may adhere through alpha 4 beta 1 integrins. In study, we demonstrate that EIIIA+, EIIIB+, V+ FN variants are synthesized, primarily by macrophages distinct temporal spatial patterns two rat cardiac transplant models: either antigenic challenge, allografts, without isografts. ratio inclusion into increases day allografts isografts high until rejected (approximately 7 days) but falls normal levels tolerated (day 6). EIIIB+ ratios peak later than do EIIIA+ FNs 4). remain relatively low Interestingly, deposited prominently myocardium rejecting close association infiltrating leukocytes, expression prominent at sites infarction. these largely restricted epicardium lesser degree immediately adjacent CS-1+ increase 3 hours after transplantation particularly days before rejection. Our data suggest differential role effector provide foundation testing their on rationale FN-based therapeutics modulate allograft recipients.