Detection and Measurement of Cardiac Ion Channels
作者： Gwilym M. Morris , Mark R. Boyett , Joseph Yanni , Rudolf Billeter , Halina Dobrzynski
DOI: 10.1007/978-1-4419-6658-2_9
关键词:
摘要: Since the 1950s, technological advances have forged molecular biology into one of most powerful fields science. The primary specializations cardiac conduction system are a lower expression fast Na+ channel (Nav1.5), background K+ (Kir2.1), and high conductance connexin (Cx43), but with higher pacemaker (HCN4) alternative L-type Ca2+ (Cav1.3). Therefore, it is possible to investigate gene transcription protein for these channels. This chapter describes use in situ hybridization, qPCR, immunohistochemistry study ion expression.
springer.com
core.ac.uk
manchester.ac.uk参考文章(20)
Arthur Smith, John Bruton, A colour atlas of histological staining techniques ,(1977)
Ellen M. Beasley, Richard M. Myers, David R. Cox, Laura C. Lazzeroni, Statistical refinement of primer design parameters PCR Applications#R##N#Protocols for Functional Genomics. pp. 55- 71 ,(1999) , 10.1016/B978-012372185-3/50006-7
Michael A. Innis, David H. Gelfand, John J. Sninsky, PCR Applications: Protocols for Functional Genomics ,(1999)
T. Brattelid, K. Tveit, J. A. K. Birkeland, I. Sjaastad, E. Qvigstad, K. A. Krobert, R. I. Hussain, T. Skomedal, J.-B. Osnes, F. O. Levy, Expression of mRNA encoding G protein-coupled receptors involved in congestive heart failure--a quantitative RT-PCR study and the question of normalisation. Basic Research in Cardiology. ,vol. 102, pp. 198- 208 ,(2007) , 10.1007/S00395-007-0648-1
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Hendrik Küpper, Laura Ort Seib, Mayandi Sivaguru, Owen A. Hoekenga, Leon V. Kochian, A method for cellular localization of gene expression via quantitative in situ hybridization in plants Plant Journal. ,vol. 50, pp. 159- 187 ,(2007) , 10.1111/J.1365-313X.2007.03031.X
Luke Pallansch, Howard Beswick, John Talian, Peggy Zelenka, Use of an RNA folding algorithm to choose regions for amplification by the polymerase chain reaction. Analytical Biochemistry. ,vol. 185, pp. 57- 62 ,(1990) , 10.1016/0003-2697(90)90254-7
W. T. ASTBURY, Molecular biology or ultrastructural biology Nature. ,vol. 190, pp. 1124- 1124 ,(1961) , 10.1038/1901124A0
James Murray, C. Elisa Oquendo, John H. Willis, Michael F. Marusich, Roderick A. Capaldi, Monitoring oxidative and nitrative modification of cellular proteins; a paradigm for identifying key disease related markers of oxidative stress. Advanced Drug Delivery Reviews. ,vol. 60, pp. 1497- 1503 ,(2008) , 10.1016/J.ADDR.2008.05.003
M. Deblock, D. Debrouwer, RNA-RNA in Situ Hybridization Using Digoxigenin-Labeled Probes: The Use of High-Molecular-Weight Polyvinyl Alcohol in the Alkaline Phosphatase Indoxyl-Nitroblue Tetrazolium Reaction Analytical Biochemistry. ,vol. 215, pp. 86- 89 ,(1993) , 10.1006/ABIO.1993.1558