Microtubule-Associated Protein 2 and Synaptophysin in the Preterm and Near-Term Ovine Fetal Brain and the Effect of Intermittent Umbilical Cord Occlusion

摘要: We have determined the change in immunoreactivity (IR) for microtubule-associated protein 2 (MAP-2) and synaptophysin (SYN) as markers dendritic presynaptic nerve development, respectively, ovine fetal brain with advancing gestation response to intermittent umbilical cord occlusion (UCO), which might then contribute adverse neurodevelopment. Fetal sheep (control experimental groups preterm at 111-115 near term 132-138 days of gestation; = 145 days) were studied over 4 UCOs performed by inflation an occluder cuff 90 seconds every 30 minutes 3 5 hours each day. Animals euthanized brains assessed IR MAP-2 SYN. In control animals, SYN increased gray matter consistent a developmental increase vesicles and/or terminals expected; however, decreased all regions studied, suggesting concurrent refinement branching spine development. Intermittent UCO marked but limited hypoxemia resulted decrease animals when turnover is higher indicates vesicle formation; whereas, was selectively hippocampus CA1 thalamus near-term reactive heightened vulnerability neuronal injury. As such, compressions fetus can impact development depending on their timing, lead alterations synapse formation circuitry.