Glycine-modified polyamidoamine dendrimers: synthesis and structural characterization using nuclear magnetic resonance, ion-mobility mass spectrometry and capillary electrophoresis
作者： Emma-Dune Leriche , Carlos Afonso , Catherine M. Lange , Martin C. Grossel , Lina Truong
DOI: 10.1039/C3RA43939A
关键词:
摘要: We present here the preparation and structural characterization of first-generation ammonia-cored polyamidoamine (PAMAM) dendrimers modified with glycine residues. Chemical modification dendrimer was done to increase biocompatibility these compounds, known be effective delivery agents for drugs or genes. Fully PAMAM [Gly6G1(N)] on one hand partially [GlynG1(N), n = 0 6)] other were obtained depending experimental conditions. The resulting have cautiously characterized understand interpret their physico-chemical biochemical properties as well control chemical design. carried out using ion mobility spectrometry-mass spectrometry (IM-MS), multistage tandem mass (MSn), accurate measurements by high resolution–mass (HR-MS), two dimensional nuclear magnetic resonance (NMR) capillary electrophoresis (CE). Characteristic fragmentation patterns compounds from ESI/MSn (with 2 4) experiments. IM-MS CE analysis showed that a single component mainly complete grafting conditions while distribution oligomers produced grafted products. physical separation GlynG1(N) oligomer ions achieved in gas phase (IM-MS) condensed Besides, collision cross sections (CCS) estimated compared theoretical values. Then, yield determined both NMR
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