Matrix-Isolated Luminescence of Primary Amines Derivatized with Naphthalenedialdehyde:

摘要: Recently a tagging reagent with superior qualities for LC analysis was developed the derivatization of primary amines which yield adducts that are highly fluorescent. This reagent, naphthalenedialdehyde (NDA), reacts in presence cyanide ion to fluorescent cyano-benz[f]-isoindole (CBI) products. High yields isoindoles formed relatively short period time when reaction is carried out at pH = 9.5. These have also been shown be stable extended periods after has initiated. Thus, NDA/CN very useful trace amines. Indeed, attomole amounts amino acids analyzed by HPLC reaction, use laser-induced fluorescence detection. Another attractive feature scheme excitation and emission maxima independent type amine solvent. In addition, long-term stability CBI-amines permits pre-column rather than post-column ortho-phthaldehyde (OPA) used as reagent.