High phosphate directly affects endothelial function by downregulating annexin II

摘要: Hyperphosphatemia is associated with increased cardiovascular risk in patients renal disease and healthy individuals. Here we tested whether high phosphate has a role the pathophysiology of events by interfering endothelial function, thereby impairing microvascular function angiogenesis. Protein expression analysis found downregulation annexin II human coronary artery cells, an effect exacerbated shedding II–positive microparticles cells exposed to media. EAhy926 sera from hyperphosphatemic also display decreased II, suggesting negative correlation between serum expression. By using cell–based assays vitro chicken chorioallantoic membrane assay vivo , that angiogenesis, vessel wall morphology, cell migration, capillary tube formation, survival were impaired milieu. Blockade membrane-bound extracellular specific antibody mimicked effects phosphate. In addition, stiffened rats . Thus, our results link adverse clinical outcomes involving endothelium both individuals disease.