Specific fractionation and manipulation of lymphocytes with derivatized nylon fibers

摘要: Abstract Antigen-coated nylon fibers have been used to fractionate and manipulate specific antigen-binding lymphocytes. Using this system, it has possible determine the frequency, specificity affinity of these cells for a variety antigens, isolate characterize antigen-specific T or B cells. An in vivo transfer system demonstrate that fractionated are highly enriched precursors antibody-secreting Experiments comparing morphology, motility immunoglobulin receptors lymphocytes bound antigen- concanavalin A-coated indicate interaction local areas on cell surface with fiber-bound ligands can alter behavior entire cell. These experiments serve sensitivity versatility antigen-coated isolating characterizing lymphoid populations. The ability visualize individual also suggests method be studies molecule-cell cell-cell interactions immune response.