Expression of 25-hydroxyvitamin D3-1α-hydroxylase in pancreatic islets

摘要: A number of studies have suggested that Vitamin D has a potential role in the development/treatment diabetes. These effects may be mediated by circulating levels 1alpha,25(OH)(2)D(3), but local production catalysed enzyme 25-hydroxyvitamin D(3)-1alpha-hydroxylase (1alpha-OHase), is also likely to important. RT-PCR analyses demonstrated both isolated rat islets and MIN6 cells (mouse insulin-secreting cell line, characteristic beta cells) expressed 1alpha-OHase mRNA. The transcript types was similar seen HKC-8 (a renal which expresses 1alpha-OHase). Western blot analysis immunolocalisation identified protein human pancreatic tissue. In addition, suspensions were able convert [3H]-25-hydroxyvitamin D(3) [3H]-1alpha,25(OH)(2)D(3), demonstrating activity. Both systems receptor 1alpha,25(OH)(2)D(3) (50nM) evoked rapid rise [Ca(2+)](i) cells. This data clearly demonstrates are produce respond rapidly treatment with 1alpha,25(OH)(2)D(3). Therefore, we would postulate maybe an important autocrine link between status function.