Tissue sections from the mature rat brain and spinal cord as substrates for neurite outgrowth in vitro: extensive growth on gray matter but little growth on white matter.

摘要: The failure of axons to regenerate within the brain and spinal cord mature mammals has been attributed absence growth-promoting substances, especially extracellular matrix components, or presence growth-inhibiting particularly components associated with CNS myelin. ability mammalian tissue support neurite regeneration was tested by growing explants embryonic chick lumbar sympathetic ganglia on fresh frozen sections rat cord. extent outgrowth quantified using morphometric analysis for grown that included most major anatomical divisions CNS. Extensive, but variable, present gray matter regions, whereas white tracts showed poor support, if any, growth. results are consistent factors also indicate regions will axonal in culture spite known components.