A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

摘要: Enzyme microreactors are important tools of miniaturized analytics and have promising applications in continuous biomanufacturing. A fundamental problem their design is that plain microchannels without extensive static internals, or packings, offer limited exposed surface area for immobilizing the enzyme. To boost immobilization a manner broadly applicable to enzymes, we coated borosilicate with silica nanosprings attached enzyme, sucrose phosphorylase, via silica-binding module genetically fused it. We showed confocal fluorescence microscopy enzyme was able penetrate ∼70 μm-thick nanospring layer became distributed uniformly Compared surface, activity immobilized enhanced 4.5-fold upon coating further increased up 10-fold by modifying sulfonate groups. Operational stability during continuous-flow biocatalytic synthesis α-glucose 1-pho...