Tear Fluid Protein Biomarkers

摘要: The tear film covers and protects the ocular surface. It contains various molecules including a large variety of proteins. protein composition fluid can change with respect to local systemic diseases. Prior advent proteomic era, analysis was limited few analytical techniques, most common which immunoelectrophoresis, an approach dependent on antibody availability. Using proteomics, hundreds proteins could potentially be identified subsequently studied. Although detection low-abundance in complex proteome remains challenge, advances sample fractionation mass spectrometry have greatly enhanced our ability detect these With increasing applications, tears show great potential as biomarkers development clinical assays for human In this chapter, we discuss structure functions methods its collection. We also summarize using techniques both Finally, modern biomarker research future challenges are explored.