Isolation and development of microsatellite loci in an African Woodpecker (Campethera nivosa) using polymerase chain reaction and DNA sequencing

摘要: The Buff-spotted Woodpecker (Campethera nivosa) is a resident bird species that distributed in lowland rainforest habitats from western to eastern Africa. We developed species-specific microsatellite markers examine the population genetics of this species. Twenty-one loci were isolated C. nivosa. Of these, 15 found amplify consistently. These then tested for variability individuals different forest localities. number alleles ranged 3 13 per locus, with observed and expected heterozygosity ranging 0.100 0.917 0.485 0.901, respectively. Four exhibited significant heterozygote deficiency while one had an excess heterozygotes. None linkage disequilibrium. polymorphic will be used study genetic populations nivosa across either sides Congo River evaluate effect river as barrier gene flow.