Effect of anchor positioning on binding and diffusion of elongated 3D DNA nanostructures on lipid membranes

摘要: DNA origami is a state-of-the-art technology that enables the fabrication of nano-objects with defined shapes, to which functional moieties, such as lipophilic anchors, can be attached nanometre scale precision. Although binding lipid membranes has been extensively demonstrated, specific requirements necessary for membrane attachment are greatly overlooked. Here, we designed set amphipathic rectangular-shaped structures varying placement and number chol-TEG anchors used attachment. Single- multiple-cholesteryl-modified nanostructures were produced studied in terms their localization, density dynamics. We show positioning at least two moieties near corners essential ensure efficient large nanostructures. Quantitative fluorescence correlation spectroscopy data further confirm increasing corner-positioned lowers dynamics flat on freestanding membranes. Taken together, our approach provides first evidence importance location addition hydrophobic when rationally designing minimal controlled binding.