Detection of glycolate oxidase gene glcD diversity among cultured and environmental marine bacteria.

作者: W. W. Y. Lau , E. V. Armbrust

DOI: 10.1111/J.1462-2920.2006.01092.X

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摘要: Of eight laboratory cultures of marine gamma- and alpha-Proteobacteria tested, growth on glycolate as a sole carbon source was detected for only three species: Pseudomonas stutzeri, Oceanimonas doudoroffii Roseobacter sp. isolate Y3F. Degenerate polymerase chain reaction (PCR) primers were designed to amplify glcD, which encodes the D-subunit enzyme oxidase; glcD could be amplified from those that grew glycolate. The PCR used explore diversity in four field samples collected different ocean environments: an Atlantic Gulf Stream Ring, sampled above below thermocline two Pacific coastal sites, Parks Bay San Juan Channel, WA. Environmental sequences belonged six major bacterial phylogenetic groups, with most forming novel clades no close relatives. Different patterns observed within between nutrient regimes. Comparison 16S rDNA analyses available genomes metgenomic library Sargasso Sea show glycolate-utilizing potential exists subset bacteria. Glycolate is produced environments mainly by phytoplankton. Examination will aid understanding influence phytoplankton community structure.

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