Expression of estrogen receptor beta in rat bone.

摘要: A novel estrogen receptor, receptor beta (ERbeta), has recently been cloned from a rat prostate cDNA library. In bone, which is an important target tissue of estrogen, ER alpha reported to be present preferentially in osteoblasts, but the mechanism action bone still not known. study, we examined expression ERbeta mRNA bone. Expression was evident primary osteoblastic cells isolated 1-day-old calvaria and osteosarcoma (ROS 17/2.8), its level higher than that mRNA. When were cultured for 28 days induce differentiation into mature osteoblasts capable forming nodules, constantly highly expressed during entire culture period. contrast, very low at beginning it gradually increased cells. Various tissues including 8-week-old rats both sexes, total RNA extracted compare distribution levels cancellous distal femoral metaphysis lumbar vertebra, obvious, equivalent those uterus testis, lower ovary prostate. The cortical There no appreciable differences between female male These results indicate suggesting there distinct mediated by