Scalable production of embryonic stem cell-derived cells.

摘要: Embryonic stem (ES) cells have the ability to self-renew as well differentiate into any cell type in body. These traits make ES an attractive "raw material" for a variety of cell-based technologies. However, uncontrolled aggregation differentiation culture inhibits proliferation and thwarts use stirred suspension bioreactors. Encapsulation agarose microdrops prevents physical interaction between developing embryoid bodies (EBs) that, turn, EB agglomeration. This enables bioreactors that can generate large numbers ES-derived under controlled conditions.