Distinct Microbiotas are Associated with Ileum-Restricted and Colon-Involving Crohn's Disease.

摘要: BACKGROUND The etiology of inflammatory bowel disease is believed to involve a shift in the microbiota toward more proinflammatory species. Crohn's (CD) usually manifests as one three phenotypes, involving inflammation terminal ileum, colon, or both. However, what determines particular phenotype and level activity remains unknown. In this study, we aim characterize intestinal associated with different CD phenotypes. METHODS DNA was extracted from biopsies 31 patients ileal, ileocolic, colon-restricted CD, also 5 non-inflammatory control subjects, analyzed by 16S rRNA gene amplicon pyrosequencing. Data were processed using Quantitative Insights Into Microbial Ecology pipeline linear discriminant analysis effect size estimation PICRUSt algorithms. Two additional recently published cohorts study. RESULTS Highly significant separation observed between bacterial composition ileal compared colonic involvement (genus Bray-Curtis P = 0.005, R 20%). This unaffected biopsy's location its state, patients' condition (remission relapse). Faecalibacterium strongly reduced involvement, whereas Enterobacteriaceae abundant former. Fusobacterium relative abundance correlated ileal-involving, but not colon-involving, CD. CONCLUSIONS Ileal colon-involving sustain distinct microbiotas, suggesting that mechanisms underlie two major manifestations potential contribution should be further investigated.