AFLP analysis of Lactuca saligna germplasm collections from four European and three Middle Eastern countries
作者： Miloslav Kitner , Aleš Lebeda , Ivana Doležalová , Marko Maras , Eva Křístková
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摘要: Lactuca saligna L. (least lettuce, willow-leaf lettuce) is widely distributed around the Mediterranean basin and extends to Caucasus temperate Europe; its extra-European distribution covers Middle East. The aim of this study was estimate level molecular variation extent genetic differentiation in accessions collected natural semi-natural habitats Europe In sum, 67 saligna, originating four European countries (Czech Republic, France, Italy, Portugal) three Eastern (Israel, Jordan, Turkey), were compared on basis AFLP polymorphism. analysis revealed 415 (84.7%) polymorphic fragments out 490 amplified by means seven primer pair combinations. number bands produced individual combinations ranged from 44 101. UPGMA dendrogram generated Jaccard's similarity matrix showed a similar background East (Turkey, Israel, Jordan), which quite distinct group part (Italy, Portugal). Surprisingly, two accessions, one Czech Republic (central Europe) proved genetically wholly separate all other are themselves separate. Accessions various eco-geographical conditions found differ significantly their
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