Antioxidant profile of nimesulide, indomethacin and diclofenac in phosphatidylcholine liposomes (PCL) as membrane model.

摘要: An in vitro assay based on the oxidation of phosphatidylcholine liposomes (PCL) with which we can distinguish anti-HO zero activity from antilipoperoxidant (R zero, ROO RO zero) has been developed for testing free-radical-scavenging ability antiinflammatory drugs. PCL were exposed to a flux hydroxyl radicals generated by water sonolysis different periods, and spontaneous lipid peroxidative phenomenon (propagation breakdown phases) was followed subsequent 24 hours. Lipid peroxidation assayed simultaneous measurements a) conjugated dienes, UV spectroscopy (absorbance second derivative at 233 nm), b) loss substrate (PC), HPLC, c) products (total carbonyl functions as 2,4-dinitrophenylhydrazones). Diclofenac, nimesulide indomethacin, added starting stages peroxidation, tested their overall specific anti-radical properties. All drugs exhibited remarkable scavenging against oxy radicals, determined percent inhibition formation concentrations easily attainable vivo (IC50:diclofenac 2.5 microM, 4.92 indomethacin 6.85 microM), diclofenac being most effective quenching R degree species responsible propagation phase. By contrast, antioxidant less potent alkyl peroxyl radical scavengers, is due restrain induction phase chain reaction mediated (IC50:nimesulide 1.85 3.57 microM).