GABA Inhibits Migration of Luteinizing Hormone-Releasing Hormone Neurons in Embryonic Olfactory Explants

摘要: During development, a subpopulation of olfactory neurons transiently expresses GABA. The spatiotemporal pattern GABAergic expression coincides with migration luteinizing hormone-releasing hormone (LHRH) from the pit to CNS. In this investigation, we evaluated role input on LHRH neuronal using explants, previously shown exhibit outgrowth axons, in association subset these and presence olfactory-derived population. GABA A receptor antagonists bicuculline (10 −5 m) or picrotoxin −4 had no effect length peripherin-immunoreactive fibers cell number. However, migration, as determined by distance immunopositive cells migrated pits, was significantly increased perturbations. Addition tetrodotoxin −6 m), inhibit Na + -transduced electrical activity, also enhanced migration. most robust observed dramatic inhibition explants cultured agonist muscimol m). This study demonstrates that activity nasal regions can have profound effects suggests participates appropriate timing into developing brain.