STUDIES ON THE SENSITIZATION OF ANIMALS WITH SIMPLE CHEMICAL COMPOUNDS : XI. THE FATE OF LABELED PICRYL CHLORIDE AND DINITROCHLOROBENZENE AFTER SENSITIZING INJECTIONS

摘要: The fate of (14)C-labeled allergens injected intradermally into guinea pigs, namely picryl chloride (PCl*) and 2:4 dinitrochlorobenzene (DNCB*), was followed during the induction period delayed hypersensitivity. Both chemicals were applied in a single injection one ear amounts that approached their minimal sensitizing doses (PCl, 0.25 microg; DNCB, 5.0 microg). Radioactivity various tissues determined by liquid scintillation counting after combustion to CO(2) H(2)O. seemed leave site three phases. A large proportion allergen escaped rapidly from ear, about 50% within 3 hr case PCl, 15 min for difference probably reflecting unequal reaction constants. Initially there "half-life" escape 2.5 with dosage microg PCl 18 DNCB. This occurred via regional veins not lymphatics. Radioactive decomposition products already present urine 3-4 hr. After 6-8 hr, half-life time lengthened approximately 28 both used respective initial dosages, holding up 2 days which still further slowing; between 4 43 much longer (72-88 hr?) apparently different physicochemical properties this second fraction. Sensitization be connected portion 12 period. It is known how far radioactivity may represent gradual hydrolysis attached dinitrophenyl groupings, respectively, form picric acid dinitrophenol. Gradual accumulation fraction lymph nodes could definitely excluded. noted no hypersensitivity arose essentially depot existed when DNCB dose microg, owing its ready ear; but 20 times as caused sensitization provided same fixed chloride. had been established, traces measurable at site. third fraction, representing coupling product, very low rate traceable through several weeks. No demonstrable detected thymus, spleen, mesenteric examined short intervals (1/2) 17 days. In analogy findings on transplantation "immunity" studies reported following paper, can explained encounters lymphoid cells hapten complex found local days, agreement Medawar's concept peripheral sensitization.