Bicarbonate secreted from the pancreas contributed to the formation of Ca precipitates in Japanese eel, Anguilla japonica.

摘要: Marine teleosts produce Ca precipitates in the intestine as a product of osmoregulation. are formed by chemical reaction Mg(2+) and Ca(2+) derived from ingested seawater with bicarbonate (HCO(3)(-)). It has been reported that HCO(3)(-) originates intestine; however, pancreas is predicted to be another organ may supply intestinal tract. In present study, was surgically removed Japanese eel confirm its contribution precipitate formation. Pancreatectomized produced significantly less than control seawater, indicating contributes substantially formation precipitates. To further examine molecular mechanisms secretion, we cloned cDNAs encoding transporters identified those elevated their mRNA expression following transfer. intestine, Slc26a6A increased shortly after transfer, whereas Slc26a1 gradually pancreas, Slc26a3 high during early stage acclimation, transfer seawater. therefore, both transient progressively increasing types likely involved secretion into lumen coordinated manner.