Manipulating yeast genome using plasmid vectors.

摘要: The vectors and techniques described here enable one to manipulate the yeast genome meet specific needs. Genes can be cloned, clone used delete wild-type gene from chromosome, or replace it with mutant versions. Mutants derived by classical methods, such as mutagenesis of whole cells, reversion a phenotype, cloned analyzed in vitro. Yeast genes foreign either inserted into autonomously replicating plasmid that are reasonably stable integrated chromosome where they maintained at copy per genome. combination these characterized promoter systems available make possible express almost any yeast. Once this is achieved, entire repertoire genetics probe function gene, engineer expression useful ways.