Hepatic total 3 alpha-hydroxy bile acids concentration and enzyme activities in prednisone-treated dogs.

摘要: High serum alkaline phosphatase (ALP) activity is considered a sensitive marker of cholestasis in most mammalian species, including dogs. Induction high ALP association with dependent on hepatic bile acids concentrations. Treatment dogs glucocorticoids also results activity. The possible causal relation between and concentration was investigated treated glucocorticoids. glucocorticoid treatment to changes the individual isoenzymes, alanine transaminase (ALT) gamma-glutamyltransferase (GGT) investigated. Eight conditioned were given 4 mg prednisone/kg body weight, i.m., daily for 10 days. Blood samples taken prior days 3, 5, 7, 10. Liver tissue then from each dog. Serum total significantly (P < 0.05) at day 3 prednisone-treated Isoenzyme analysis indicated that this increase attributable an liver isoenzyme (LALP). Significant increases corticosteroid-induced (CALP) bone first observed 7 10, respectively. ALT GGT activities increased by 5. Increased or concentrations not dogs, compared values 8 clinically normal (control) but complete duct ligation. Hepatic LALP, CALP, higher than controls, indicating probable synthesis these enzymes. increased.(ABSTRACT TRUNCATED AT 250 WORDS)