A transcriptomic analysis of bermudagrass (Cynodon dactylon) provides novel insights into the basis of low temperature tolerance

摘要: Cold stress is regarded as a key factor limiting widespread use for bermudagrass (Cynodon dactylon). Therefore, to improve cold tolerance bermudagrass, it urgent understand molecular mechanisms of response stress. However, our knowledge about the responses this species largely unknown. The objective study was characterize transcriptomic low temperature in by using RNA-Seq platform. Ten cDNA libraries were generated from RNA samples leaves five different treatments cold-resistant (R) and cold-sensitive (S) genotypes, including 4 °C acclimation (CA) 24 h 48 h, freezing (−5 °C) 4 h with or without prior CA, controls. When subjected acclimation, global gene expressions initiated more quickly R genotype than those in the S genotype. activated expression effectively after 48 h CA than the differentially expressed genes identified sensing signaling-related genes, functional proteins transcription factors, many which were specifically predominantly under treatments, implying that these play important roles enhanced hardiness bermudagrass. KEGG pathway enrichment analysis DEGs revealed photosynthesis, nitrogen metabolism carbon fixation pathways results may contribute understanding mechanism underlying stress, also provide clues further in-depth characterization cold-resistance breeding candidate