The Effect of HRE-Regulated VEGF Expression and Transfection on Neural Stem Cells in Rats.
作者： Bo Dou , Xiangrong Zheng , Danfeng Tan , Xixi Yin
DOI: 10.3389/FCELL.2020.580824
关键词:
摘要: In this study, we analyzed neural stem cells transfected with the HRE-VEGF gene in groups experiencing different periods of hypoxia. The results RT-PCR showed that expression vascular endothelial growth factor (VEGF) mRNA gradually increased prolonged period hypoxia (p < 0.05). from western-blot test VEGF protein as MTT combined Elisa reagent hypoxia, secretion increased, and proliferation target was better promoted These imply HRE can safely effectively regulate expression. By controlling increase level, limit it more safe values to avoid possibility cancer caused by over-enhancement due overexpression protein.
frontiersin.org
sci-hub.st 参考文章(11)
Chinmay K. Mukhopadhyay, Barsanjit Mazumder, Paul L. Fox, Role of Hypoxia-inducible Factor-1 in Transcriptional Activation of Ceruloplasmin by Iron Deficiency Journal of Biological Chemistry. ,vol. 275, pp. 21048- 21054 ,(2000) , 10.1074/JBC.M000636200
Xiang-rong Zheng, Shan-shan Zhang, Fei Yin, Jie-lu Tang, Yu-jia Yang, Xia Wang, Le Zhong, Neuroprotection of VEGF-expression neural stem cells in neonatal cerebral palsy rats Behavioural Brain Research. ,vol. 230, pp. 108- 115 ,(2012) , 10.1016/J.BBR.2012.01.026
Weibang Liang, Qianqian Han, Wei Jin, Zhifeng Xiao, Jingchun Huang, Hongbin Ni, Bing Chen, Jie Kong, Jun Wu, Jianwu Dai, The promotion of neurological recovery in the rat spinal cord crushed injury model by collagen-binding BDNF. Biomaterials. ,vol. 31, pp. 8634- 8641 ,(2010) , 10.1016/J.BIOMATERIALS.2010.07.084
Zheng Gang Zhang, Li Zhang, Quan Jiang, Ruilan Zhang, Kenneth Davies, Cecylia Powers, Nicholas van Bruggen, Michael Chopp, VEGF enhances angiogenesis and promotes blood-brain barrier leakage in the ischemic brain Journal of Clinical Investigation. ,vol. 106, pp. 829- 838 ,(2000) , 10.1172/JCI9369
Ling Wei, Zheng Z. Wei, Michael Qize Jiang, Osama Mohamad, Shan Ping Yu, Stem cell transplantation therapy for multifaceted therapeutic benefits after stroke Progress in Neurobiology. ,vol. 157, pp. 49- 78 ,(2017) , 10.1016/J.PNEUROBIO.2017.03.003
Alissa C. Greenwald, Tamar Licht, Saran Kumar, Sunday S. Oladipupo, Seema Iyer, Myriam Grunewald, Eli Keshet, VEGF expands erythropoiesis via hypoxia-independent induction of erythropoietin in noncanonical perivascular stromal cells. Journal of Experimental Medicine. ,vol. 216, pp. 215- 230 ,(2019) , 10.1084/JEM.20180752
Xuri Li, Xiaodong Sun, Peter Carmeliet, Hallmarks of Endothelial Cell Metabolism in Health and Disease. Cell Metabolism. ,vol. 30, pp. 414- 433 ,(2019) , 10.1016/J.CMET.2019.08.011
Tomás Sobrino, Manuel Rodríguez-Yáñez, Francisco Campos, Ramón Iglesias-Rey, Mónica Millán, Natalia Pérez de la Ossa, Antonio Dávalos, Raquel Delgado-Mederos, Alejandro Martínez-Domeño, Joan Martí-Fábregas, Mar Castellanos, Joaquín Serena, Aida Lago, Exuperio Díez-Tejedor, José Castillo, Association of High Serum Levels of Growth Factors with Good Outcome in Ischemic Stroke: a Multicenter Study Translational Stroke Research. ,vol. 11, pp. 653- 663 ,(2020) , 10.1007/S12975-019-00747-2
Anita C. Truttmann, Vanessa Ginet, Julien Puyal, Current Evidence on Cell Death in Preterm Brain Injury in Human and Preclinical Models. Frontiers in Cell and Developmental Biology. ,vol. 8, pp. 27- ,(2020) , 10.3389/FCELL.2020.00027
Ping Sun, Kai Zhang, Sulaiman H. Hassan, Xuejing Zhang, Xuelian Tang, Hongjian Pu, R. Anne Stetler, Jun Chen, Ke-Jie Yin, Endothelium-Targeted Deletion of microRNA-15a/16-1 Promotes Poststroke Angiogenesis and Improves Long-Term Neurological Recovery Circulation Research. ,vol. 126, pp. 1040- 1057 ,(2020) , 10.1161/CIRCRESAHA.119.315886