Variable performance of a human derived Sarcoptes scabiei recombinant antigen ELISA in swine mange diagnosis

摘要: Abstract The performance of an indirect ELISA test based on Sarcoptes scabiei var hominis recombinant antigen Ssλ20ΔB3 (rec-ELISA), to diagnose pig mange was investigated in 15 experimentally infected and non-infected pigs 692 commercial from 16 herds southeast Spain. These latter animals included 6–7 month old fatteners (13 herds), 11–12 replacement sows (1 herd) ≥24 breeding (7 herds). All were examined for mites ear skin scrapings the presence S. scabiei-associated macroscopic dermatitis; moreover, also tested antibodies against porcine viruses including: Aujeszky disease virus (ADV), swine influenza (SIV), type 2 circovirus (PCV2) respiratory reproductive syndrome (PRRSV). chronic hyperkeratotic dermatitis detected 6 herds. Mite prevalence other 83% sows, 0% 7 fattener's 3–82% fattener had with acute hypersensitivity percentage affected lesion area significantly greater ones. Rec-ELISA relative optical densities (RODs) older than young pigs, as well compared pigs. However, RODs differed between individuals, regardless age origin (commercial or experimental) herd scabiei. Low repeatability microtiter plates, suggesting variable specific antibody binding antigen, are likely partly responsible ROD variation. Other potential causes variation using random effects logistic regression analysis, after defining a seropositivity threshold value receiver-operating characteristics (ROC) analysis. model indicated that associated large areas only low PCV2 seroprevalence. Pigs more extensive may have infections rec-ELISA detectable antibodies. possibility PCV2, recognized immunosupressor, depresses production infection merits further attention. In summary, results indicate some studied complementary tool herd-level diagnosis, work reduce internal external sources assay is essential.