Distribution of an Ankyrin-repeat Protein on the Endoplasmic Reticulum in Arabidopsis
作者： Liqin Wei , Yan Li
DOI: 10.1111/J.1744-7909.2008.00791.X
关键词:
摘要: There are many ankyrin-repeat proteins in plant cells. However, the distribution and function of these mostly unclear. By reverse transcription-polymerase chain reaction, a gene encoding an ankyrin-like protein was cloned from Arabidopsis named AtANK1 (GenBank accession no. NM_120340). The 6-His-tagged AtAnk1-N fusion affinity-purified its rabbit polyclonal antibody obtained. Immuno-blotting with purified anti-AtAnk1-N revealed that relative molecular weight about 76 kDa. immunofluorescence labeling immuno-gold antibody, coupled confocal transmission electron microscopy observation, found to be distributed on membrane endoplasmic reticulum Based results, we suggested might involved reticulum-related localization sorting
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