Genetic and biochemical analysis of the adenylyl cyclase-associated protein, cap, in Schizosaccharomyces pombe.

摘要: We have identified, cloned, and studied a gene, cap, encoding protein that is associated with adenylyl cyclase in the fission yeast Schizosaccharomyces pombe. This shares significant sequence homology cyclase-associated CAP Saccharomyces cerevisiae. bifunctional protein; N-terminal domain appears to be involved cellular responsiveness RAS, whereas loss of C-terminal portion morphological nutritional defects. S. pombe cap can suppress phenotypes deletion cerevisiae but does not domain. Analysis disruptants also mapped function two domains. The functional region results abnormal morphology, slow growth, failure grow at 37 degrees C. Increases mating sporulation were observed when entire gene was disrupted. Overproduction both highly elongated large cells are sterile measurably higher levels activity. Our indicate required for proper has other functions shared CAP.