Rat angiotensinogen is secreted only constitutively when transfected into AtT-20 cells.

摘要: To test whether angiotensinogen might be targeted to dense core secretory granules in cells containing a regulated pathway, we expressed rat AtT-20 cells, mouse pituitary cell line that has the demonstrated ability correctly sort proteins constitutive or pathway. We compared pattern of secretion with endogenous adrenocorticotropin hormone, which is secreted by through When were incubated for 5 hours dibutyryladenosine cyclic monophosphate KCl, hormone was significantly higher than control, whereas monensin had no effect. In contrast, markedly reduced monensin, but stimulation observed KCl. These results make it unlikely could cotargeted active renin Alternative mechanisms must explain how angiotensin II synthesized locally tissue renin-angiotensin systems.