Comparison of fluorescence-labeling strategies of colon cancer for fluorescence-guided surgery of liver metastasis in orthotopic mouse models

摘要: Abstract The goal of three studies was to determine if fluorescence-guided surgery (FGS) could be an improvement over standard bright-light (BLS) in experimental colon cancer liver metastasis nude mouse models. In one study orthotopic model human HT-29-green fluorescent protein (GFP)-expressing established, and mice were randomized into FGS or BLS groups. performed using a handheld portable fluorescence imaging system visualize the during surgery. FGS-treated had significantly prolonged disease-free survival (DFS) overall (OS) compared BLS-treated mice. another study, we used telomerase-dependent GFP-containing adenovirus OBP-401 label situ model. OBP-401-GFP-labeled completely resected with FGS. contrast, not effect complete resection. OBP-401-FGS reduced recurrence rate OS BLS. expressing GFP subsequently labeled anti-CEA antibody conjugated DyLight 650. Mice underwent anti-CEA-DyLight that smaller area residual tumor longer DFS OS. This series experiments obtained models suggest can benefit resection this recalcitrant disease clinic.